The MTDF is a world-class high-throughput eukaryotic protein production and purification facility. I implemented a pipeline for expression of mammalian proteins, the majority of which were secreted, glycosylated members of the immunoglobulin superfamily. The pipeline resulted in small scale expression screening of 3000 baculoviral constructs in two years, more than 1200 large scale (multi liter) production runs of insect cells, and 900 large scale purifications. The high-throughput nature of this expression pipeline necessitated a high degree of automation; I established protocols for baculovirus generation on a custom cell::explorer system, and wrote protocols automating mammalian and general cell culture procedures. We have successfully purified hundreds of milligrams of high-quality, endotoxin-free protein, for pre-clinical testing.
The expertise of the Almo lab in structural determination and immunology, in conjunction with our protein production and purification abilities, is applied to a range of exciting projects within the group. These projects range from the development of an epitope specific immunomodulation platform, a potential biotherapeutic, to platforms for screening ligand-receptor interactions.